Eggs, vitamins, color, and fluorescence all play a part in this classroom demonstration by Prof. Hal White of the University of Delaware. The demonstration i...

Comment

You need to be a member of PULSE to add comments!

Join PULSE

Comment by Dr Henry Furneaux on June 3, 2013 at 1:17pm

yup, indeed , lots of potential , I will be trying it out in the fall semester !

Will let you know what happens !

best

Henry

Comment by Harold B. White on June 3, 2013 at 1:08pm

Henry,Approaching this from a nuitional perspective works well. Chick embryos and humans need similar nutrients to grow and survive. The great feature of riboflavin is that you can actually see the vitamin in normal egg white. In the absence of mutant eggs, you could show normal eggs and then tell the story about eggs that have clear colorless egg whites and don't hatch and have students propose explanations.

Hal

Comment by Dr Henry Furneaux on June 3, 2013 at 11:20am

Hi . Many thanks for the rapid  feedback.  I was thinking of presenting the biology first , these eggs develop , these do not . Then asking the students to figure it out ( with a little prompting) . So they would want to look in the egg and would discover the  difference.  I would forsee that several lab exercises would be needed to answer the question .   If the eggs  are not readily available , I could  provide egg white . Using affinity chromatography , i could create egg white that lack riboflavin and that lacks the binding protein and would be a mimic.

Providing  them with the sequence of the w.t. and mutant genes  might be a great way for them to discover splicing !

Thanks for your help

best

Henry

Comment by Harold B. White on June 3, 2013 at 11:08am

Henry,

I no longer do research on the riboflavin-deficient strain of chickens and it is no longer maintained here at the University of Delaware. Marilee Benore at the University of Michigan Dearborn, may be able to get you some eggs. The riboflavin-binding protein-deficiency in eggs is caused by a splicing mutation that results in a null allele, no protein is produced. [J. Biol. Chem. 268, 23222-23226 (1993)] The wild type protein is very easy to purify from chicken egg white where it constitutes about 0.5% of the protein and binds very strongly to DEAE cellulose. [Meth. Enzymol. 122, 227-234 (1986)] Details of the demonstration have been published. [ J. Chem. Educ. 65, 814-815 (1988)] and a laboratory experiment for a biochemistry course has been published [J. Chem. Ed. 67, 803-804 (1990)].   I doubt that a recombinant protein is available or would be desirable since there are a number of post-translational modifications to the protein. [Ann. Rev. Nutr. 8, 279-299 (1988)]

While the demonstration is great with a supply of eggs from mutant hens, that is icing on the cake. Because the demonstration involves familiar things--eggs, vitamins, color, and fluorescence, it can be easily modified and retain student interest. For a little twist, you might want to include duck eggs which have the riboflavin-binding protein in their egg white, but there is no yellow color because there is virtually no riboflavin present. The demonstration works best in a totally darkened room and a good UV light source.

Good luck.

Hal White

Comment by Dr Henry Furneaux on June 3, 2013 at 9:46am

 I want to  do  this.

Where can you get  the  eggs that lack the binding protein ?

What's the mutation  ?

 (mutation in regulatory region or  deletion?   or is it an amino acid change in protein ) ?

Is purified (or recombinant )  binding protein available ? This would enable adding it  back    to restore quenching .

Presumably it can be purified using a riboflavin  affinity column ?

Latest Activity

Shelley Haydel joined David Marcey's group
Thumbnail

Southwest PULSErs

Please share this group with others This group is intended as a networking site for PULSE community members from the US Southwest and Hawaii. Please add ideas for regional conferences, let your regional colleagues know about your PULSE-related activities, etc. If you would like to be contacted in the future in order to get involved in specific initiatives, please fill out the recruitment form. If you know of someone who isn't yet a member of this group, please feel free to invite them.See More
2 hours ago
Shelley Haydel is now a member of PULSE
3 hours ago
Anjali Misra replied to David Marcey's discussion Call for Active Learning Resources in the group Transforming the Traditional Lecture
"Here is the URL to place the order. This is $ 50.00 only. http://www.flinnsci.com/store/Scripts/prodView.asp?idproduct=22433"
4 hours ago

Leadership Fellow
David Marcey replied to David Marcey's discussion Call for Active Learning Resources in the group Transforming the Traditional Lecture
"Thanks, Anjali, can you give contact info for ordering?"
5 hours ago
Anjali Misra replied to David Marcey's discussion Call for Active Learning Resources in the group Transforming the Traditional Lecture
"Hi David, I have been using process oriented guided inquiry learning (POGIL) biology activities in my classes for almost a year now. These activities foster the peer learning environment in the class and I have observed dramatic improvement in…"
Sunday
Anjali Misra joined David Marcey's group
Thumbnail

Transforming the Traditional Lecture

This group is for anyone interested in brainstorming methods to convert traditional lectures to arenas of inquiry where active learning takes place. See More
Sunday
Anil Kumar Challa replied to David Marcey's discussion Ambassador Interest in the group Spreading the PULSE
"Anil Kumar Challa Department of Genetics University of Alabama at Birmingham 660 KAUL 720 20th Street South Birmingham, AL 35294 Office: (205) 934 7212"
Sunday
Ana Maria Barral joined Edwin Vázquez's group
Thumbnail

Transforming the laboratory exercises into research experiences

At UPR-Cayey we are trying to substitute the traditional recipe-type laboratory exercises with research projects that have the elements of scientific inquiry.  One idea is to identify and define two or three semester-long research projects that are easy to do in terms of cost-effectiveness, materials, etc. Students would work in groups of 4-5 and present their results in a seminar at the end of the semester.  There would be lab meetings, article discussions, etc.We plan to pilot-test this idea with two lab sections (approx. 16 students each) of General Biology I next year (2013).  Among the challenges we may face are how to scale-up, the nature of the projects, integration with the lecture, and assessment, among others.Any ideas, comments and suggestions are welcome.  See More
Saturday

Leadership Fellow
Akif Uzman liked Ana Maria Barral's discussion Yale's Small World Initiative: looking for partners
Saturday

Leadership Fellow
David Marcey replied to Ana Maria Barral's discussion Yale's Small World Initiative: looking for partners in the group Southwest PULSErs
"Thanks, Anna Maria! I will pass this on to our Microbiologist and Intro lab director. David "
Saturday
Ana Maria Barral added a discussion to the group Southwest PULSErs
Thumbnail

Yale's Small World Initiative: looking for partners

Dear SW PULSE members,One of the VC recommendations is to increase student participation in research, especially for undergraduates. In 2013 Yale launched the Small World Initiative (SWI), where students collect soil samples and isolate antibiotic producing bacteria. The framework can be adapted not only for a microbiology course, but also molecular biology and general biology.In its 2nd year of implementation, only us (National University) and CSU Humboldt (this summer, not started yet) are partners in California. Pilot results indicate the framework can be adapted to a number of course setups and schedules. Yale provides lab manual material (which can be adapted), protocols, as well as free sequencing of PCR products for partners.Yale has held 2 onsite trainings, but I think the future will be more e-training and direct regional networking. Our school has implemented SWI in Costa Mesa and San Diego. I am looking for faculty in the region interested in becoming involved, as the…See More
Saturday
Anil Kumar Challa posted a status
Friday
Anil Kumar Challa joined Nitya Jacob's group
Thumbnail

Spreading the PULSE

Welcome PULSE Community Members! We Want You! ... to serve as ambassadors that will spread V&C nationally. If you are familiar with V&C reforms, literature that supports the implementation of such reforms, and share a passion for spreading the word at a national level, you are an ideal candidate for serving as a PULSE V&C Ambassador.The “Spreading the Pulse” working group is focused on building multiple teams of PULSE Ambassadors (visitation teams) equipped with a V&C reform toolkit (see "Toolkit" discussion, below).  The role of the Ambassadors will be to meet with biology and life science  departments to encourage them to adopt the principles and recommendations of the “Vision and Change” report. The toolkit will include resources, references, strategic planning templates, and other items. We are…See More
Friday
Profile IconKristopher Wright and Anil Kumar Challa joined PULSE
Thursday
Anil Kumar Challa joined David Marcey's group
Thumbnail

Transforming the Traditional Lecture

This group is for anyone interested in brainstorming methods to convert traditional lectures to arenas of inquiry where active learning takes place. See More
Thursday
Anil Kumar Challa joined Edwin Vázquez's group
Thumbnail

Transforming the laboratory exercises into research experiences

At UPR-Cayey we are trying to substitute the traditional recipe-type laboratory exercises with research projects that have the elements of scientific inquiry.  One idea is to identify and define two or three semester-long research projects that are easy to do in terms of cost-effectiveness, materials, etc. Students would work in groups of 4-5 and present their results in a seminar at the end of the semester.  There would be lab meetings, article discussions, etc.We plan to pilot-test this idea with two lab sections (approx. 16 students each) of General Biology I next year (2013).  Among the challenges we may face are how to scale-up, the nature of the projects, integration with the lecture, and assessment, among others.Any ideas, comments and suggestions are welcome.  See More
Thursday

© 2014   Created by Kathryn G. Miller.   Powered by

Badges  |  Report an Issue  |  Terms of Service