Eggs, vitamins, color, and fluorescence all play a part in this classroom demonstration by Prof. Hal White of the University of Delaware. The demonstration i...


You need to be a member of PULSE to add comments!


Comment by Dr Henry Furneaux on June 3, 2013 at 1:17pm

yup, indeed , lots of potential , I will be trying it out in the fall semester !

Will let you know what happens !



Comment by Harold B. White on June 3, 2013 at 1:08pm

Henry,Approaching this from a nuitional perspective works well. Chick embryos and humans need similar nutrients to grow and survive. The great feature of riboflavin is that you can actually see the vitamin in normal egg white. In the absence of mutant eggs, you could show normal eggs and then tell the story about eggs that have clear colorless egg whites and don't hatch and have students propose explanations.


Comment by Dr Henry Furneaux on June 3, 2013 at 11:20am

Hi . Many thanks for the rapid  feedback.  I was thinking of presenting the biology first , these eggs develop , these do not . Then asking the students to figure it out ( with a little prompting) . So they would want to look in the egg and would discover the  difference.  I would forsee that several lab exercises would be needed to answer the question .   If the eggs  are not readily available , I could  provide egg white . Using affinity chromatography , i could create egg white that lack riboflavin and that lacks the binding protein and would be a mimic.

Providing  them with the sequence of the w.t. and mutant genes  might be a great way for them to discover splicing !

Thanks for your help



Comment by Harold B. White on June 3, 2013 at 11:08am


I no longer do research on the riboflavin-deficient strain of chickens and it is no longer maintained here at the University of Delaware. Marilee Benore at the University of Michigan Dearborn, may be able to get you some eggs. The riboflavin-binding protein-deficiency in eggs is caused by a splicing mutation that results in a null allele, no protein is produced. [J. Biol. Chem. 268, 23222-23226 (1993)] The wild type protein is very easy to purify from chicken egg white where it constitutes about 0.5% of the protein and binds very strongly to DEAE cellulose. [Meth. Enzymol. 122, 227-234 (1986)] Details of the demonstration have been published. [ J. Chem. Educ. 65, 814-815 (1988)] and a laboratory experiment for a biochemistry course has been published [J. Chem. Ed. 67, 803-804 (1990)].   I doubt that a recombinant protein is available or would be desirable since there are a number of post-translational modifications to the protein. [Ann. Rev. Nutr. 8, 279-299 (1988)]

While the demonstration is great with a supply of eggs from mutant hens, that is icing on the cake. Because the demonstration involves familiar things--eggs, vitamins, color, and fluorescence, it can be easily modified and retain student interest. For a little twist, you might want to include duck eggs which have the riboflavin-binding protein in their egg white, but there is no yellow color because there is virtually no riboflavin present. The demonstration works best in a totally darkened room and a good UV light source.

Good luck.

Hal White

Comment by Dr Henry Furneaux on June 3, 2013 at 9:46am

 I want to  do  this.

Where can you get  the  eggs that lack the binding protein ?

What's the mutation  ?

 (mutation in regulatory region or  deletion?   or is it an amino acid change in protein ) ?

Is purified (or recombinant )  binding protein available ? This would enable adding it  back    to restore quenching .

Presumably it can be purified using a riboflavin  affinity column ?

Latest Activity

Profile IconCatherine Loudon and Regina Schurman joined PULSE
7 hours ago
Profile IconMonica Tischler, Robert McCarthy, Cheryl Heinz and 1 more joined PULSE
Erin McKenney is now a member of PULSE
Sunita Yadav replied to David Marcey's discussion Ambassador Interest in the group Spreading the PULSE
"Dear Sir, I am interested and would be like to join. Kindly find my contact details mentioned below:  Thanks and Regards, Sunita Yadav Senior Research Fellow Advanced Technology Development Centre Indian Institute of Technology Kharagpur…"
Profile IconGretchen Edwalds-Gilbert and Scott Woody joined PULSE
Aug 15

Leadership Fellow
David Marcey commented on Dan (A. Daniel) Johnson's blog post How Do Textbooks Need to Change?
"Dan, I believe the future lies in content delivered via creative, multimedia video, with texts employed as references. "
Aug 15
Patrick Raymond Clark posted a page
Aug 14

Leadership Fellow
Karen Klyczek liked Kathryn G. Miller's group Midwest-Great Plains Regional Network
Aug 13
Patrick Raymond Clark liked Sharon Gusky's discussion Greatest Challenges to the Implementation of the Vision and Change Recommendations
Aug 12
Profile IconPatricia Robison and Leslie Lane joined PULSE
Aug 12
Profile IconRobin Rylaarsdam and Amy Mulnix joined PULSE
Aug 11
Profile IconTeresa Balser (Teri), Sandra L. Romano and 4 other members joined Nitya Jacob's group

Spreading the PULSE

Welcome PULSE Community Members! We Want You! ... to serve as ambassadors that will spread V&C nationally. If you are familiar with V&C reforms, literature that supports the implementation of such reforms, and share a passion for spreading the word at a national level, you are an ideal candidate for serving as a PULSE V&C Ambassador.The “Spreading the Pulse” working group is focused on building multiple teams of PULSE Ambassadors (visitation teams) equipped with a V&C reform toolkit (see "Toolkit" discussion, below).  The role of the Ambassadors will be to meet with biology and life science  departments to encourage them to adopt the principles and recommendations of the “Vision and Change” report. The toolkit will include resources, references, strategic planning templates, and other items. We are…See More
Aug 9
Mary Shaw joined David Marcey's group

Southwest PULSErs

Please share this group with others This group is intended as a networking site for PULSE community members from the US Southwest and Hawaii. Please add ideas for regional conferences, let your regional colleagues know about your PULSE-related activities, etc. If you would like to be contacted in the future in order to get involved in specific initiatives, please fill out the recruitment form. If you know of someone who isn't yet a member of this group, please feel free to invite them.See More
Aug 9

Leadership Fellow
Karen Klyczek posted an event

National Association of Biology Teachers Professional Development Conference at Cleveland Convention Center

November 12, 2014 at 1pm to November 15, 2014 at 3pm
Join other leaders in biology and life science education for four days of renowned speakers, hands-on workshops, 200+ informative sessions and special events!PULSE Fellows will be presenting several sessions, including a pre-conference workshop on Wed 11/12, 1-4:00, Faculty as Change Agents: Facilitating Departmental Transformation Toward Vision & Change.  Additional sessions include Challenging Students (and Us) to Listen, Evaluate, and Think Creatively, and an update on PULSE programs and products.See More
Aug 8
Rachel Horak is now a member of PULSE
Aug 7
Profile IconChrisha L Dolan and Melissa Zwick joined PULSE
Aug 5

© 2014   Created by Kathryn G. Miller.   Powered by

Badges  |  Report an Issue  |  Terms of Service